INFORM October 2025

inform October 2025, Vol. 36 (9) • 35

Evaluation of culture conditions of Trichosporon oleaginosus DSM11815 for enhancement of growth and lipid production in sugarcane bagasse hydrolysate as a substrate of Trichosporon oleaginosus DSM11815 were optimized, and the potential of sugarcane bagasse hydrolysate as an alternative carbon source for culturing the oleaginous yeast was evaluated. The fermentation process in the potato glucose agar (PDA) medium was optimized through single-factor experiments, including initial total sugar concentration, nitrogen source type, carbon nitrogen ratio (C/N ratio), KH 2 PO 4 concentration, fed-batch period, and composition of the fed-batch solution. In the optimized PDA medium, which contained 20 g/L glucose, 7.5 g/L xylose, 0.51 g/L urea, 0.2 g/L KH 2 PO 4 , the two-stage culture through fed batch with T. oleaginosus DSM11815 led to the production of dry weight of 28.2 g/L with the lipid content of 41.1 %. In comparison to the groups of the optimized PDA medium and sugarcane bagasse hydrolysate, the group that utilized sugarcane bagasse hydrolysate to replace half of the optimized PDA medium obtained the highest biomass (39.4 g/L), the highest lipid con tent (50.00 %), the highest glucose consumption (100 %) and the Fang, Y., et al. , Process Biochemistry , 155, 23, 2025. https://doi.org/10.1016/j.procbio.2025.04.015 In this study, the growth and lipid production

successive germination and sourdough fermentation processes can further boost the content of particular nutrients and sensory attributes of the enriched bread.

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Thais Lomonaco Teodoro da Silva teaches and conducts food sci ence research at the Federal University of Lavras (UFLA), Brazil. Her research is focused on oleogels, lipid crystallization, and sonocrystallization.

Oleaginous yeasts are emerging as a promising platform for the production of single-cell oil. Their remarkable capacity to accumu late high lipid concentrations makes them attractive for industrial applications. A key advantage lies in their ability to utilize agricul tural waste streams as low-cost substrates, transforming these discarded materials into valuable products. This bio-upcycling strat egy, powered by microbial fermentation, not only addresses waste management but also establishes a sustainable and economically viable route for lipid synthesis. The resulting microbial lipids can serve as a renewable alternative to traditional oils, with potential applications in biofuels, oleochemicals, and edible applications.